Modelo do resumo

THERAPEUTIC DRUG MONITORING OF CEFEPIME, VANCOMYCIN AND IMIPENEM IN BURN PATIENTS BY SIMULTANEOUS DETERMINATION USING HPLC-UV

KARIN JANNET VERA LÓPEZ, DANIELLE FERREIRA FARIA BERTOLUCI, KARINA MARIA VICENTE, ADRIANA MARIA DELL AQUILA, SILVIA REGINA CAVANI JORGE SANTOS

FCF USP – SP.

Introduction and objective: Cefepime(C), vancomycin (V) and imipenem (I) are antimicrobial agents commonly prescribed to burn patients with sepse. Therapeutic drug monitoring for dose adjustment of C, V and I in large burn patients by HPLC-UV and also pharmacokinetics are investigated.
Materials and methods: Venous blood (2ml ) was collected from femoral catheter, transferred to tubes containing sodium EDTA. Plasma was obtained;1mL was transferred to an Eppendorf tube (2mL) containing 0.25mL of MOPS solution 10%, then samples were stored at –80º C. Two burned patients were investigated, #A, male, 31yr, 175cm, 75 kg , total burned BSA of 45%, V (1g, one hr infusion every 12hr) and C (2g, 10min. infusion every 8 h). Patient #B, male, 47yr, 168cm, 56 kg , with burns on 40% of his BSA, V(1g, one hour infusion every 12hr) and I (1g infusion, 15min. every 6 h). A serial of six blood samples (2 mL/each) of both patients were withdrawn, samples were stored at –80º C until assay. Analytical method required only 200 µL of plasma, the clean up of biological matrices was performed by precipitation of plasma proteins followed by an HPLC-UV method reported previously for the simultaneous analysis of these antibiotics.
Results and conclusion: Plasma levels of C, I and V in two burn patients indicated that therapeutic drug monitoring is pharmacoeconomically viable, since burn patients were exposed to subtherapeutic blood levels, increasing the risk of bacterial resistance. Pharmacokinetic data were included and discussed for all drugs investigated.
Support or studentship: Fapesp, CNPq.